The use of DNA amplification fingerprinting (DAF) as a tool for monitoring mixed microbial populations in bioreactors was evaluated. Short (8-mer or mer). PDF | DNA amplification fingerprinting (DAF) is a strategy for genetic typing and mapping that uses one or more very short (≥5 nt) arbitrary oligonucleotides to. Identification of DNA Amplification Fingerprinting (DAF) markers close to the symbiosis-ineffective sym31 mutation of pea (Pisum sativum L.).

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There is not such a requirement for daf.

Another genetic approach has recently been suggested by two groups 5amplificatiom in which PCR amplification of DNA polymorphisms was directed by single ajplification primers only eight to 10 nucleotides in length.

Application of the polymerase chain reaction to the diagnosis of human genetic disease. Please review our privacy policy. U se of the polymerase chain reaction pcramplification of targeted nucleotide sequences as a means of identifying microorganisms for diagnostic and epidemiologic purposes 12has initiated a transformation in laboratory based approaches to infectious disease diagnosis and was the subject of an earlier LCDC report 3.

The polymerase chain reaction. The polymerase chain reaction: A new method of using molecular genetics for medical diagnosis. Fingerprinting amplififation using PCR with arbitrary primers.


DNA amplification fingerprinting using arbitrary oligonucleotide primers.

National Center for Biotechnology InformationU. The high sensitivity and specificity of properly targeted long chain primer pairs and the great variety of specimens amenable to PCR analysis, including nonculturable specimens such as paraffin embedded tissues, has generated numerous diagnostic applications 4.

Following polyacrylamide gel electrophoresis pagethese demonstrate an array of amplicons that yield a banding fingerprint that is characteristic for each combination of short primer and DNA source. This technique has been designated DNA amplification fingerprinting daf. Amplificatiob polymorphism amplified by arbitrary primers are useful as genetic markers.

DNA diagnostics — Molecular techniques and automation. Particularly important are the specialized silver staining fngerprinting 8 required for resolution of small, as opposed to large, amplicons, and the primer concentrations required for effective priming.

The conditions required for effective long chain, dual primer PCR are considerably more stringent with respect to hybridization and enzymatic conditions than for daf.

Until then it would seem reasonable to predict that daf technology will provide high versatility while retaining the selectivity and raf of long, dual primer PCR and will shortly be part of the diagnostic trade of clinical microbiologists.

DNA amplification fingerprinting: Another diagnostic tool?

Reiss J, Fingerprintimg DN. Can J Infect Dis. Welsh J, McClelland M. There appears to be some particular advantages to daf compared to the more traditional PCR approach.


DNA amplification fingerprinting using arbitrary oligonucleotide primers.

Support Center Support Center. Fast and sensitive silver staining of DNA in polyacrylamide gels.

LCDC has begun the analysis of certain pseudomonads using this technology and we will shortly be reporting on its efficacy in our hands. The use of long, dual primer PCR requires that there be extensive preexisting knowledge of the target sequences.

Multiple priming sites on each DNA strand are amplified efficiently by short primers under relaxed conditions and the number of amplicons reproducibly obtained in daf is largely dependent on the short primer sequence rather than length, provided the number exceeds four 5 — 7. An overview and development of diagnostic protocols at the LCDC. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers.

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