Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs This paper describes the development, optimization and performance assessment of a rapid and highly sensitive PCR test for detection of pseudorabies virus.
PRV specific primers were designed using the Oligo 6.
The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected.
Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed.
Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR. The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine.
Doença de Aujeszky
The effect doeha temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction. Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. Finally, nucleic rm from tissue homogenates samples derived from seven healthy pigs, and a non infected PK cell line were also tested showing no positive products data not shown.
Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I. The viral agent following a primary replication can establish latent infection and develops a latency-reactivation infection which allows its perpetuation in aujezzky populations 1012 This assay was based on the amplification of a highly suino viral gD gene fragment.
Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4. The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and downa disorders in adults 28 Seven tissue samples suinoz clinically healthy animals were negative for PCR amplification data not shown.
Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2.
Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs. National Center for Biotechnology InformationU.
In addition, positive amplifications were obtained in all the tissue samples, dona PRV natural infected pigs, evaluated. The PCR assay described here provides a rapid, highly sensitive, and cost-effective laboratory diagnosis for pseudorabies infections.
Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier.
Published online Sep 1. Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
Thus, the optimal concentration of MgCl2 and primers were 1. Author information Article notes Copyright and License information Disclaimer. A rapid, sensitive and specific PCR-based diagnostic xuinos to detect pseudorabies virus in clinical samples is provided. Support Center Support Center.
Iowa State University Press; Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes. In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders. All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis.
The analytical sensitivity of the test was consistently observed to be 1.